Now there is an alternative method of preparing human sperm for IUI – the PureSperm SpeediKit. The kit contains sufficient sperm preparation materials for ten patients, already dispensed in Type 1 glass centrifuge tubes.

• Sodium chloride
• Potassium chloride
• Magnesium sulphate
• Potassium dihydrogen phosphate
• Sodium bicarbonate
• EDTA
• hSA (human Serum Albumin)
• Sodium citrate
• Glucose
• Calcium lactate
• Sodium pyruvate
• HEPES
• Purified water
• Silane-coated silica

Performance Characteristics

• pH of PureSperm UniLayer: 7.4 – 7.8.
• pH of PureSperm Wash: 7.3 – 8.5.
• Osmolality of PureSperm UniLayer (mOsm/kg H2O): 300 – 330.
• Osmolality of PureSperm Wash (mOsm/kg H2O): 290 – 300.
• Endotoxin levels (EU/mL): <1.0.
• Contents are tested by human sperm survival only.
• Tubes and stoppers are M.E.A. tested.

• Store at 2 to 30ºC and avoid temperatures above or below these values. Under these conditions the contents of the kit have a shelf-life of minimum 12 months from production. The expiry date is shown on both tubes and cartons. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
• No antibiotics, unstable additives or preservatives have been added by the manufacturer to PureSperm SpeediKit.

• When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination.
• Use aseptic procedures at all times.
• If available, use sealed buckets during centrifugation to avoid creation of aerosols.
• Clean accidental spills using a dampened cloth or paper. PureSperm SpeediKit causes floors and benches to be extremely slippery.
• PureSperm SpeediKit does not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.
• Do not use any solution which shows evidence of bacterial contamination.
• Do not use contents if tamper-evident seal is broken.
• Federal Law (USA) restricts this device to sale by or on the order of a physician.
• Please check for regulatory compliance governing the use of ART products in your country.

It can be extremely difficult to obtain good yields of motile sperm from highly viscous semen samples. Nidacon has developed a method that could be helpful to you in the lab. You simply add PureSperm Buffer to the ejaculate, incubate at 37°C for 15-30 minutes and the sample is ready to use. This will give you a much higher proportion of motile sperm.

Protocol: 
For sperm preparation from a viscous semen sample:

1. Bring all solutions to room temperature (17 to 27°C).
2. Measure the volume of the semen sample.
3. Dilute 1+2 , 1 part PureSperm Buffer and 2 parts semen sample
(e.g. 0.5 ml PureSperm Buffer + 1.0 ml semen sample).
4. Incubate at 37°C for 15-30 minutes.
5. Mix using a pipette.
6. Ready for sperm preparation on a density gradient.

Article No. / Name / Volume

PSSK-010 / PureSperm SpeediKit / 10 patient preps

*  Orders exceeding 30 pcs, need to be order 3 months prior to delivery.

SDS-PSW – View PDF
SDS – PSUL – View PDF

Product insert (latest version) – View PDF

For other languages, contact Nidacon

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