• Colloidal silica particles coated with silane, in an isotonic salt solution.
• Unopened bottles have a shelf-life of 2 years.
• pH of 7.4 – 7.8 at room temperature and at 37°C.
• Low endotoxin levels.
• Terminally sterilised by autoclaving.
•  Bottles are made of borosilicate glass and silicone stoppers with tamper-evident seals.
• Bottles and stoppers have been M.E.A. tested.

• Silane-coated silica
• Calcium chloride
• Potassium chloride
• Sodium chloride
• Purified water
• HEPES
• EDTA
• Glucose

• Store unopened bottles at 2 – 40°C, avoid temperatures above or below these values. Under these conditions BoviPure or BoviDilute have a shelf life of 2 years. The expiry date is shown the labels.
• Open and close bottles under aseptic conditions. After opening, store the bottles at 2 – 8°C.
• No antibiotics, unstable additives or preservatives have been added to BoviPure or BoviDilute.

• When retrieving the sperm pellets, follow the instructions given in the product insert to avoid inadvertent re-contamination.
• To ensure best possible results use aseptic conditions at all times.
• Use sealed buckets or centrifuge tubes during centrifugation to avoid the creation of aerosols.
• Clean accidental spills with dampened cloth or paper. BoviPure cause floors and bench tops to be slippery.
• Neither BoviPure nor BoviDilute represent any fire or combustion hazard. A material safety data sheet is available from the manufacturer or distributor.

IFU

SDS

Article No. / Name / Volume
BP-100 / BoviPure / 100 mL

A comparison of BoviPure and Percoll on bull sperm separation protocols for IVF.

Samardzija M, Karadjole M, Matkovic M, Cergolj M, Getz I, Dobranic T, Tomaskovic A, Petric J, Surina J, Grizelj J, Karadjole T.
Anim Reprod Sci. 2006 Feb;91(3-4):237-47
Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
“Our results indicate that both protocols gave suitable sperm for IVF. This finding and the similarity between those two density gradient preparations suggests that BoviPure is a good alternative for sperm separation in bovine IVF”

Effects of bovine spermatozoa preparation on embryonic development in vitro.

Samardzija M et al
Reprod Biol Endocrinol. 2006 Nov 13;4 (1):58 17101040
“So, we concluded that BoviPure® could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos”.

Retained Functional Integrity of Bull Spermatozoa after Double Freezing and Thawing Using PureSperm® Density Gradient Centrifugation.

WMC Maxwell 1 , I Parrilla 2 , I Caballero 2 , E Garcia 2 , J Roca 2 , EA Martinez 2 , JM Vazquez 2 and D Rath 3
Reproduction in Domestic Animals, 2007 Oct, Volume 42 Issue 5, Pages 489 – 494
“These results demonstrate that bull semen can be frozen and thawed, followed by a second freeze-thawing cycle of a population of spermatozoa selected by PureSperm®, with retained motility and functional integrity. This points to the possibility of using double frozen spermatozoa in bovine artificial insemination programmes and to the potential benefits of PureSperm® density gradient centrifugation for the application of cryopreserved bull spermatozoa to other biotechnological procedures such as flow cytometric sex sorting followed by re-freezing and thawing.”

Develompent of Procedures for Sex-Sorting Frozen-Thawed Bovine Spermatozoa.

Underwood et al
Reproduction in Domestic Animals, 2009 Jun;44(3):460-6. Epub 2008 Nov 2
“In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.

BoviPure Pro

Effect of Density Gradient Centrifugation with Trypsin on the Fertilizing Capability of Bovine Sperm .

B. A. Blevins, M. de la Rey and N. M. Loskutoff
Reproduction, Fertility and Development 2008;20(7): 784 – 8
“In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.